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Gram Positive Bacteria vs Gram Negative Bacteria

August 29, 2019

Hello and welcome to Biology Professor. Today, we will be talking about one way that you can distinguish most types of bacteria. Most bacteria fall into one of two categories. Gram-Positive or Gram-Negative So first, let’s talk about the Gram-Positive type of bacteria. The way they are distinguished is between differences in their cell walls. With the Gram-Positive cell wall, Tte two most important features are a thick layer of peptidoglycan and one membrane. Let’s look at this in a little bit more depth. This thick layer of peptidoglycan is important for structure of the cell and for helping the bacteria cell maintain its shape. Then you have this inner wall zone that is between the thick peptidoglycan layer and the single membrane. Within this plasma membrane, you have two main components. These are the lipids which are shown here, a lipid bilayer, and also several different types of proteins. I’ll mark these with an asterisk (*) each. So these are all proteins that serve different functions within that plasma membrane. Finally there is one other distinguishing characteristic that you see in Gram-Positive cell walls and this is what I have indicated here with these blue lines. These are called lipoteichoic acids and these anchor through the peptidoglycan layer onto lipids in this lipid bilayer. So these are lipoteichoic acids. And now if we look these are some common examples of Gram-Positive bacteria. That I am sure you have heard of Staphylococcus aureus can actually be part of the normal human bacterial flora. Actually, a majority of humans have staph in their nose and on the skin. But it can also cause disease. Specifically some strains of Staph. aureus can cause food poisoning and they can also cause infections of the skin and of the respiratory tract. There is also Bacillus anthracis which causes Anthrax. Clostridium tetani which is responsible for Tetanus. and Streptococcus mutans this is usually associated with cavities in the mouth.
So, this is the structure of a Gram-Positive cell wall. So now we are going to continue our conversation about the difference between Gram-Positive and Gram-Negative cell walls. This time looking at Gram-Negative cell wall. We still see some of the same components. There is still a plasma membrane. It’s still composed of a lipid bilayer and it still contains proteins. That I will label here with an asterisk (x). There is still a layer of peptidoglycan, but you can see that compared to Gram-Positive peptidoglycan layer, in Gram-Negative cells, this peptidoglycan layer is much, much thinner. and also unique to Gram-Negative cell walls, this peptidoglycan layer is anchored to an outer membrane, so a second lipid bilayer, with additional proteins. Now in the outer membrane there are some proteins that are called porins. These porins allow for passive diffusion of things from the outside into the space between the two membranes, which is called the periplasmic space. These porins allow most things to diffuse through as long as the can fit. There is nothing specific about that. The actual selective permeability of the cell is still controlled by this inner plasma membrane. Now another very important feature of Gram-Negative cell walls is what is on the very outside of the this outer membrane. LPS, or lipopolysaccharides, this a component that is only found on the outside of Gram-Negative cell walls. and that has very important consequences when you have infections of Gram-Negative bacteria in the blood. This LPS, you will also hear it referred to as endotoxin. for exactly that reason. Now lets look at some examples of Gram-Negative bacteria. You have probably heard of a lot of these. E. coli is one that we hear about a lot as a pathogen because it can cause food poisoning. But it is actually a normal commensal part of the human flora. So it is not always pathogenic. There is also Helicobacter pylori, which causes ulcers and stomach cancer. Vibrio cholerae, which causes cholera. Yersinia pestis, which causes plague. This is the bacteria that is responsible for the historically famous Black Death. Also, Salmonella enterica, which is another common cause of food poisoning. So that concludes our explanation of the differences between Gram-Positive cell walls and Gram-Negative cell walls. If you are interested in more information about how these two types of bacteria are distinguish between when diagnosing illnesses or when studying them in the laboratory, please see my video on the Gram stain procedure and also thank you for watching.


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    Awesome vid, very helpful. Thanks

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    It helped me a lot, tks. The lecture is very clear, easy to understand that helps me much in study.

  • Reply Brandon Payne May 28, 2014 at 2:00 am

    Gram negative!!!!

  • Reply Amir Translateur June 22, 2014 at 12:32 am

    thank you for your video! 🙂 (Btw, I think it's Vibrio cholerae)

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    Love your video! Can you please upload more videos? It so helpful =)

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    thanks a lot ,very helpful :)))

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    very helpful. Thanks a lot

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    se nota que esta bien explicado, lo malo  que no se mucho ingles

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    simple to digest

  • Reply Amin Ali October 27, 2014 at 8:20 am

    thanks alot

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    thank you very much! 

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    thanks, i love your video

  • Reply Aisosa Ihama December 16, 2014 at 2:20 am

    Does anyone know which of the two types is most susceptible to antibodies? I thought it would be gram-positive bacteria because of the thicker peptidoglycan layer and the lack of porins…

  • Reply Reginald Finley December 28, 2014 at 6:41 pm

    You rock. Thanks!

  • Reply james lombardo January 15, 2015 at 8:43 pm

    Great explanation of this..I didn't think it was going to be a great explanation on Youtube..Nice work.

  • Reply Matthew Zelig January 26, 2015 at 3:14 am

    Thank you for the help, I appreciate it

  • Reply Alan Avila February 8, 2015 at 7:03 am

    Excellent video, very well explained, and really simple.

  • Reply Jonathan VanName March 5, 2015 at 6:00 am

    For some reason, people are having a difficult time understanding why gram positive bacteria is more receptive to antibiotics than gram negative. Even though gram positive has that thick peptidoglycan layer, it lacks that outer membrane that the gram negative has. Thus, it takes a rather strong antibiotic to fully penetrate the membrane of the gram negative bacteria and to completely thwart its process.

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    This video helped me so much. Thank you for posting

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    Great! so simple but very mean full and helpful.

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  • Reply Ashish Shrestha August 21, 2015 at 10:38 am

    why some bacteria are gram positive while others are gram negative?

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    You just made this so FREAKING easy, thank you!

  • Reply Gerald Hawkins February 14, 2016 at 9:42 pm

    Very nice quick summary! To the point. I'm trying to understand better how taking (or not taking) the Gram stain affects the resistance antibacterials, and it seems the double wall of the Gram negative may make it tougher to kill. But from the bacteria's standpoint, does the double wall create constraints, like slower absorption of gases and nutrients, or make the cell less responsive to stimuli?

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    thank you very much your very easy to understand and you break things down well. thanks a bunch

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    This was so helpful! Clear and understandable.

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    Thank you very much it was very useful and please audiences do not use course language in your comments. thanks

  • Reply Tu mi May 7, 2016 at 3:57 pm

    i just love how you pronounced the bacteria without stress

  • Reply Lisa Blechynden May 23, 2016 at 2:07 am

    Audio is the most important thing. Get a better mic.

  • Reply luis vargas June 12, 2016 at 8:46 am

    Gracias por el video.

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    you are getting fat eat less lipids food

  • Reply Allan Ochoa August 31, 2016 at 2:11 am

    came here not knowing the difference between Gram positive and gram negative bacteria,
    and now i am leaving with knowledge of gram-positive and gram negative bacteria 🙂

  • Reply Masood Saleem August 31, 2016 at 11:57 pm

    Great video, excellent explanation.
    One think u need is a voice cancellation mic =)

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    Um yes…. I wish I could give you a big hug cause this condensed everything I need to know nicely.

  • Reply Haneen Hassan October 25, 2016 at 1:14 pm

    Why the endospore found in gram posative bacteria only????please answer me..i have exam in this question tomorrow???

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    great video, very clear expanation

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    great video, just underline the bacteria names

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    Hi. Very useful vid. Just cleared my head about this topic. Thanks for putting out this content which I am sure will be useful to many others. I know it was a big help for me.

  • Reply Levi Ferrell February 23, 2017 at 5:35 pm

    Great video

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    Mam i am from india
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    what about peptide linkage ?

  • Reply Ali Ahmad March 25, 2017 at 12:13 pm

    Mam i am from india
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    what about peptide linkage ?

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    when bacterial cell wall burst they will die why?

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    Sir heavy gram negative baccili infection h..but Sir ye bimari thik ho skti h ya Ni or thik ho skti h to kaise treatment hain…. I hope app meri help krenge reply me…..

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  • Reply nissim baraness January 11, 2019 at 4:33 am

    This is a VERY basic demonstration of the differences between Gram + and Gram -. tetra-peptides are the cross links between NAG and NAM that make up the peptidoglycan cell wall, Teichoic acid are also found only in Gram + although its function is not well understood. on the other end, lipoteichoic acid, in some bacteria, is made out of 60% wax, which is able to prevent the cell from drying out (making it more difficult or water to escape). As for Gram -, lipopolysaccharide, is made out of sugar monomers covalently bound to the phospholipid bilayer whose hydrophilic head are the endotoxins she was talking about.

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