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DNA replication in Prokaryotes 1 | Prokaryotic DNA replication initiation

August 19, 2019

DNA replication will be the procedure for
producing 2 equivalent reproductions collected from one of first DNA molecule. This kind
of natural method takes place in all residing organisms which is the basis intended for
natural monetary gift. DNA comprises of 2 strands in addition to each and every follicle
from the first DNA molecule acts being a web template to the manufacturing from the secondary
follicle, an operation called semiconservative replication. Cell phone proofreading in addition
to error-checking things ensure around great fidelity intended for DNA replication. In a very mobile, DNA replication commences
on unique places, or perhaps sources involving replication, within the genome. Unwinding
involving DNA at the origins in addition to activity involving completely new strands
brings about replication forks increasing bidirectional through the origins. Many proteins
are generally associated with the replication fork which often assists when it comes to
the particular initiation in addition
to continuation involving DNA activity. Almost all conspicuously, DNA polymerase synthesizes
the newest DNA with the addition of secondary nucleotides on the web template follicle.
DNA replication can also be performed throughout vitro (artificially, outside the house the
cell). DNA polymerases remote through cells in addition to man-made DNA primers can often
initiate DNA activity on regarded sequences in a very web template DNA molecule. The actual
polymerase cycle response (PCR), a typical clinical method, cyclically does apply such
man-made activity for you to increase a selected goal DNA fragment at a pool area involving
DNA. The particular initiation associated with
DNA replication can be mediated by DnaA, the healthy proteins which binds into a region
with the foundation known as the actual DnaA package. In Elizabeth. coli, you will discover
four DnaA cardboard boxes, every one of which usually boasts a remarkably conserved 9 bp
general opinion sequence 5′ — TTATCCACA — 3′. Joining associated with DnaA to this particular
region will cause that being in a wrong way supercoiled. Third ,, a region associated
with OriC upstream with the DnaA cardboard boxes (known seeing that DnaB boxes) grow
to be melted. There are three of those locations, and every single can be 13 bp prolonged, and
AT-rich (which helps shedding since
a smaller amount energy is necessary to bust the 2 hydrogen provides which type
between A new and Testosterone levels nucleotides). That region gets the general opinion sequence
5′ — GATCTNTTNTTTT — 3′. Burning with the DnaB cardboard boxes involves ATP (which
can be hydrolyzed by DnaA). Subsequent shedding, DnaA utilizes the hexameric helicase (six
DnaB proteins) to be able to complete opposite finishes with
the melted DNA. This is where the replication
derive may type. Recruitment associated with helicase involves six DnaC meats, every one
of which can be attached with one subunit associated with helicase. The moment this
kind of sophisticated can be created, an extra several DnaA meats emergency for
the authentic several DnaA meats in order
to create several DnaA dimers. DnaC can be subsequently unveiled, plus the prepriming
sophisticated can be finish. In order for DNA replication to continue, SSB healthy proteins
is necessary to stop the sole strands associated with
DNA from being created almost any second houses
and also to reduce them from reannealing, and
DNA gyrase is necessary to alleviate the load (by producing damaging supercoils)
produced by the actual motion associated with DnaB helicase. The
particular relaxing associated with DNA
by DnaB helicase makes for primase (DnaG) and RNA polymerase
to be able to perfect every single DNA theme
to ensure that DNA activity may initiate.


  • Reply Asma Alvi November 10, 2017 at 1:50 pm

    Thanks Bro. You are great!!

  • Reply SAIMA TAHSEEN HAWALDAR November 21, 2017 at 2:51 pm

    thank you sir

  • Reply Baha Sanginov November 21, 2017 at 8:35 pm

    Your lectures are easy to follow. Thank you sir.

  • Reply Jyoti Thakur November 22, 2017 at 2:39 am

    what is the function of topoisomerase during initiation process in prokaryotes

  • Reply Maria Orsic November 25, 2017 at 1:27 am

    Finally, a true teacher has arrived!!! My professors suck! They are little more than technicians. Thank you for explaining that the tension( negative supercoil) resolves itself by "melting the DNA" upstream. This is JUST one example of Shomu's gift for explaining the essence of complicated mechanisms.

  • Reply Nikita Jangra December 6, 2017 at 5:01 am

    Reallly awesome sir… πŸ‘Œ

  • Reply Pooja Singla December 6, 2017 at 10:46 pm

    thankyou soo much for the video.. this is really helpful…

  • Reply Prajna Pradhan December 8, 2017 at 8:22 am

    sir please make a video on chromosome condensation

  • Reply Meghna sharma December 9, 2017 at 8:34 am

    Life saviour.
    Thanks a ton.

  • Reply Jessica Baa December 23, 2017 at 5:21 pm

    My doubts are getting solved…these videos are of great help!

  • Reply Shivangi Singh Gautam January 8, 2018 at 4:45 pm

    How can u be soooooo intelligent…. ?😍😍😍😍

  • Reply Toha Ariyan January 16, 2018 at 3:17 pm

    Really helpful sir.

  • Reply Rumana Tamboli January 31, 2018 at 12:41 am

    The best online teacher

  • Reply Dinesh Lee February 2, 2018 at 1:56 am

    Nice teaching bro.. I can easily understand …this video… Thank u..

  • Reply Shiwani kapoor February 20, 2018 at 7:42 am

    Just a amazing video ,it helps me a lot thank you

  • Reply Mahima Divekar February 24, 2018 at 10:54 am

    Knowledge given by you is perfect bt it takes too much time as each video is of half hr for a single concept ..πŸ˜‘

  • Reply Mamung Hage March 6, 2018 at 4:40 pm

    Is it possible to get your contact details?

  • Reply Joydeep Ghosal March 20, 2018 at 5:47 am


  • Reply TEJASWINI TAJANE March 27, 2018 at 12:24 pm

    Nice seminar in Initiation of DNA Replication in prokaryktic

  • Reply Sumakshu Ghodeswar March 28, 2018 at 3:41 pm

    u are a boon for biology students. keep it up sirπŸ‘

  • Reply Vikas Sharma March 29, 2018 at 4:04 am

    Ultimate bro…

  • Reply Vikas Sharma March 29, 2018 at 4:04 am

    Lubb uhh… Suprb teaching

  • Reply Sima Ahmeda March 30, 2018 at 6:53 am

    why initiation start only S phage.. plzz sir give me ans ?

  • Reply Sima Ahmeda March 30, 2018 at 6:53 am

    why initiation start only S phage.. plzz sir give me ans ?

  • Reply Rudolf Gyorkei March 31, 2018 at 9:12 pm

    Shomu, create a video about why mules are fertile

  • Reply E Vision April 4, 2018 at 2:59 am

    Thank you…..

  • Reply Rohan Avinash Phatak April 5, 2018 at 7:12 am

    U go soo out of the track. Really man. 13 bp 3 seq ka point hone k bad tum kaha Pohoch gaye. Plz explain to the point and by considering an average student's perspective

  • Reply Shrawan Yadav April 5, 2018 at 3:00 pm

    somu sir have u arranged ur all videos systematically.i am doctor.i really like ur videos.i suggest u to arrange ur all videos urself.please don't let other to do this.

  • Reply StereoSoundAgent April 11, 2018 at 1:56 pm

    do you have a pet bird in the background?

  • Reply Mehmet Azwar April 15, 2018 at 4:11 pm


  • Reply Diptimayee Biswal April 23, 2018 at 2:22 pm

    So DNA helicase nd topoisomerase enzyme is not needed here ?

  • Reply kashish kamra April 24, 2018 at 5:19 pm

    awesome sir

  • Reply Soulx 21 April 25, 2018 at 2:12 pm

    DNA C stays in the complex after the attachment of the complex in the strand? And what is the direction of The complex unwinding???
    Awesome lesson
    thanks Sir.

  • Reply evneet kaur bhatia April 29, 2018 at 11:07 am

    Very well explained….Thank you

  • Reply Mubarak Dar May 4, 2018 at 3:24 pm

    Really great sir!!!!I m extremely thankful to u sir…

  • Reply Sayan Khan May 6, 2018 at 2:14 pm

    the proteins used in this process are not DNA A or DNA B, they are Dna A and Dna B.

  • Reply Dangerous. Prince May 13, 2018 at 2:36 pm

    Apki awaz bht ahista hai Thora loud bolen to it will make easy for me to listen….. otherwise ur videos r bestest

  • Reply Avina kotangale May 23, 2018 at 5:25 pm

    Please improve the voice quality

  • Reply sebastian kora June 1, 2018 at 8:24 am

    This was so cool. Like my sir can't teach so much without creating ruckus of chaos

  • Reply Aniket Aniket June 8, 2018 at 6:06 pm

    Wow bro πŸ‘πŸ‘

  • Reply Aniket Aniket June 8, 2018 at 6:14 pm

    Please you trying a cell divition chapt…😊

  • Reply Priya June 10, 2018 at 5:20 pm

    Sir, this video is very good, but the audio is bit low. we usually watch your video in classroom, that is noisy. so, please consider this if possible.

  • Reply Ava Flynn June 16, 2018 at 8:30 am

    thank u so much Sir …😊😊

  • Reply Shama Kausar June 28, 2018 at 3:51 pm

    what is the use of topoisomerase

  • Reply PANKAJ MOHANTA July 21, 2018 at 2:36 am

    Sir thanks very much. There is 9 base pair long, 5 sequences given in Life Sciences book

  • Reply supriya pathak August 15, 2018 at 3:45 pm

    U made this topic very simple for a better understanding, great way of teaching😍

  • Reply supriya pathak August 15, 2018 at 3:58 pm

    Helicase 5'to3' badhta hπŸ˜‘

  • Reply Sudipan Roy August 23, 2018 at 6:35 am

    Thank you Sir.

  • Reply Ganesh Ram August 27, 2018 at 4:38 am

    one mistake is that there is 5 set of 9bp.

  • Reply Parath Sahu August 28, 2018 at 12:50 am

    Sir Dna G protein ka exact function kya hua?
    Pls explain sir

  • Reply fortune nyamutswa September 20, 2018 at 7:01 pm

    good lecturing but I feel like you have to talk about initiation first before mixing up with everything

  • Reply Parul Sharma September 24, 2018 at 6:15 pm

    Sir pls send me this video in hindi

  • Reply Jairah B. September 30, 2018 at 2:32 pm

    please put some subtitles, some of the words are inaudible

  • Reply Alwina Anam October 9, 2018 at 12:29 pm

    One of the best teacher…. Thank you so much…

  • Reply Alwina Anam October 9, 2018 at 12:30 pm

    Please make ur live videos on plant biotechnolgy

  • Reply Pankaj Behera October 12, 2018 at 9:20 pm

    Describe Kinds of innate behavior with e.g video korie brother

  • Reply Asha H M October 19, 2018 at 3:29 am

    I have doubt G /C rich ratio where present we can break bond easily because there is 3 hydrogen bonds between them.

  • Reply Waqar Ahmad October 19, 2018 at 4:51 pm

    play with 1.5 speed. thank me later. its a wonderful video

  • Reply Alex Rayan October 25, 2018 at 7:01 pm

    bro i want dna replication and its types ,events and enzmes in replication

  • Reply Indhira kumar October 30, 2018 at 10:36 am

    Which component or enzyme cause the DNA bubble

  • Reply Maria Shabir October 31, 2018 at 5:20 pm

    Ua oll time great bro….. keep spreading this knowledge… i mean the way you frame ua lecture .. its oustanding…… … future genetiscst

  • Reply shalu tyagi November 1, 2018 at 12:51 pm

    amazing video sir

  • Reply Sushree Shatarupa November 1, 2018 at 3:07 pm

    But there are 5 9mer motif

  • Reply Arjun Pal November 8, 2018 at 12:56 pm

    sir can you please make a video on the important parasites of man. quite a many qustions are being asked regarding parasites and the diseases they cause.

  • Reply Akshit Jain November 17, 2018 at 6:27 am

    Can you add the links of videos on DNA polymerase and other enzymes required in dna replication in the description.

  • Reply Soumallya Sen November 19, 2018 at 5:19 am

    There is 9 nucleotide repeats in 5 numbers..

  • Reply golden otis November 30, 2018 at 5:28 am

    Ok who is that person trying so hard to breathe?

  • Reply Neha pandey December 5, 2018 at 9:38 am

    Nice explanation sir…thanku very much

  • Reply Satya December 7, 2018 at 9:28 am

    the style of talking and personality is so amizing…

  • Reply Riddhi Pitliya Jain December 15, 2018 at 3:30 am

    at around 21m, you talk about DNA-B and how it forms the replication bubble by breaking/melting the H-bonds. but why do we need 2 sets of DNA-B (+DNA-C) complexes to attach to both strands? wouldn't one complex suffice in separating the strands as breaking the h-bonds from one strand's nucleotide bases should be enough?

  • Reply Sree Sree December 16, 2018 at 6:15 am

    Thankyou so much sir

  • Reply LAV Maithani December 22, 2018 at 1:07 pm

    I can't get the idea of Ori and RRS , its confusing .
    You say Ori is in upstream of RRS
    but. I think RRS are generally located in upstream
    May be I am wrong

  • Reply Rohit Kadam December 24, 2018 at 5:51 am

    There are 11 9bp recognition sequences…

  • Reply Rohit Kadam December 24, 2018 at 5:53 am

    Completely wrong information for initiation!

  • Reply Abhinandan Dhar December 25, 2018 at 10:11 am

    Nice sir keep it up… And it would be helpful if u coould please give a sum up of the topic in the end of your upcoming lectures it would b very helpful….

  • Reply Subhojit Bag December 26, 2018 at 3:17 pm

    It's very helpful

  • Reply Dashrth Bhaliya January 1, 2019 at 10:01 am

    9 base pair repeats are 5 consecutive repeats..Not 4

  • Reply Ajay Rathore January 21, 2019 at 4:16 pm


  • Reply sai divakar sanjeev Yanamadala January 24, 2019 at 1:01 pm

    bahubali ending at last

  • Reply Aatif Jan January 27, 2019 at 5:12 am

    Thank u

  • Reply Shreya Alva February 2, 2019 at 9:57 am

    Thank you, this is very helpful

  • Reply priya Jungari February 10, 2019 at 3:34 pm

    very nice video sir… Thank u so much..

  • Reply Sudhanya Dutta February 13, 2019 at 7:34 pm

    Sir rna polymerase?

  • Reply ritu singh March 1, 2019 at 2:00 pm

    Thank you so much

  • Reply Aasif Amin March 1, 2019 at 7:04 pm

    plz make video on transposons

  • Reply Jason Clark March 7, 2019 at 5:32 pm

    what can I say??? you are nice man, niiiiiice

  • Reply shobha surbhaiyya March 9, 2019 at 5:06 pm

    nice explanation……

  • Reply Rashmi Jha March 28, 2019 at 10:43 am

    Can you please provide notes on this topic . πŸ™πŸ™

  • Reply Muhammad Abbas April 11, 2019 at 7:05 pm

    Good job sir

  • Reply Pradip sharma April 12, 2019 at 12:49 pm

    Sound quality is not quite good

  • Reply Banjara tv April 15, 2019 at 1:57 pm

    Recognition 9 bp sequence 5 units not 4

  • Reply taniats73 May 5, 2019 at 5:55 am

    Thank you sir..thank you very much..

  • Reply Neha Sharma June 3, 2019 at 8:51 am

    Sir thank u….its was very easy to understand….as i got strucked in few point….with the diagrammatic presentation it was too easyfor me to understand…

  • Reply Reynold George June 18, 2019 at 2:21 pm

    😊 thank you

  • Reply Netaji Gudenkwar June 22, 2019 at 3:57 pm

    Nice lecturer

  • Reply Dua Ihsan June 24, 2019 at 8:08 am

    God bless you

  • Reply Bikram Roy July 4, 2019 at 10:55 am

    You r great sir❀❀❀ tumi sera sir

  • Reply farishna karki July 6, 2019 at 4:01 am

    Your videos are really amazing πŸ‘. . .

  • Reply Prateek panda July 8, 2019 at 3:52 pm

    Awesome πŸ‘πŸ‘πŸ‘πŸ‘πŸ‘

  • Reply kabelo Makgoba July 27, 2019 at 4:03 am

    Dude! You're the manπŸ‘πŸ‘Œ.

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